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Literature summary extracted from

  • Johansson, N.; Persson, K.; Norbeck, J.; Larsson, C.
    Expression of NADH-oxidases enhances ethylene productivity in Saccharomyces cerevisiae expressing the bacterial EFE (2017), Biotechnol. Bioprocess Eng., 22, 195-199 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.19 DNA and amino acid sequence determination and analysis, sequence comparisons with Pseudomonas digitatum and Pseudomonas chrysogenum, recombinant expression in Saccharomyces cerevisiae Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 DNA and amino acid sequence determination and analysis, sequence comparisons with Pseudomonas digitatum and Pseudomonas chrysogenum, recombinant expression in Saccharomyces cerevisiae Pseudomonas syringae pv. pisi

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.19 A199G site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 A199G site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 C280F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 C280F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 D191A site-directed mutagenesis, inactive mutant Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 D191A site-directed mutagenesis, inactive mutant Pseudomonas syringae pv. pisi
1.13.12.19 E235D site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 E235D site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 F278Y site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 F278Y site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 H189A site-directed mutagenesis, inactive mutant Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 H189A site-directed mutagenesis, inactive mutant Pseudomonas syringae pv. pisi
1.13.12.19 H233A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 H233A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 H268A site-directed mutagenesis, inactive mutant Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 H268A site-directed mutagenesis, inactive mutant Pseudomonas syringae pv. pisi
1.13.12.19 I254M site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 I254M site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 I304N site-directed mutagenesis, inactive mutant Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 I304N site-directed mutagenesis, inactive mutant Pseudomonas syringae pv. pisi
1.13.12.19 I322V site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 I322V site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 L22M site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 L22M site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 additional information a loop deletion mutant is inactive Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 additional information a loop deletion mutant is inactive Pseudomonas syringae pv. pisi
1.13.12.19 R236S site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 R236S site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 V172T site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 V172T site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pseudomonas syringae pv. pisi
1.13.12.19 V212Y/E213S site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 V212Y/E213S site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pseudomonas syringae pv. pisi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.19 Fe2+ dependent on, residues H189, D191 and H268 are responsible for binding the Fe(II) ligand Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 Fe2+ dependent on, residues H189, D191 and H268 are responsible for binding the Fe(II) ligand Pseudomonas syringae pv. pisi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2 Pseudomonas savastanoi pv. phaseolicola
-
 ethylene + 3 CO2 + H2O
-
 ?
1.13.12.19 2-oxoglutarate + O2 Pseudomonas syringae pv. pisi
-
 ethylene + 3 CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.19 Pseudomonas savastanoi pv. phaseolicola P32021
-
-
1.13.12.19 Pseudomonas syringae pv. pisi Q9Z3T0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2
-
 Pseudomonas savastanoi pv. phaseolicola ethylene + 3 CO2 + H2O
-
 ?
1.13.12.19 2-oxoglutarate + O2
-
 Pseudomonas syringae pv. pisi ethylene + 3 CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.19 EFE
-
 Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 EFE
-
 Pseudomonas syringae pv. pisi
1.13.12.19 ethylene forming enzyme
-
 Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 ethylene forming enzyme
-
 Pseudomonas syringae pv. pisi

General Information

EC Number General Information Comment Organism
1.13.12.19 evolution the enzyme belongs to a subclass of 2-oxoglutarate/Fe(II) dependent dioxygenases, structure-function analysis of the ethylene forming subclass of 2-oxoglutarate/Fe(II)-dependent dioxygenases, overview Pseudomonas syringae pv. pisi
1.13.12.19 evolution the enzyme belongs to a subclass of 2-oxoglutarate/Fe(II) dependent dioxygenases, structure-function analysis of the ethylene forming subclass of 2-oxoglutarate/Fe(II)-dependent dioxygenases,overview Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 additional information three of the amino acids correlating with ethylene production are located in the predicted 2-oxoglutarate binding domain, a protein domain specific for the EFE-class that is essential for activity. Residues H189, D191 and H268 are responsible for binding the Fe(II) ligand Pseudomonas savastanoi pv. phaseolicola
1.13.12.19 additional information three of the amino acids correlating with ethylene production are located in the predicted 2-oxoglutarate binding domain, a protein domain specific for the EFE-class that is essential for activity. Residues H189, D191 and H268 are responsible for binding the Fe(II) ligand Pseudomonas syringae pv. pisi